Dionex AminoPac PA1 氨基酸分析柱，主要用于氨基酸的快速分离。非常适用于分析酸性和酸不稳定性氨基酸，以及用阳离子交换分离不完全的氨基酸对。
Dionex AminoPac PA1 氨基酸分析柱
|AminoPac PA1 Analytical Column (4 x 250 mm)||037022|
|AminoPac PA1 Guard Column (4 x 50 mm)||037023|
For Specialized Amino Acid Applications with Postcolumn Derivatization
The AminoPac® PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.
Resolves phosphorylated and other amino acids by anion-exchange chromatography
Separates acid-labile amino acids under basic pH conditions
Separates strongly acidic amino acids
Requires OPA or ninhydrin postcolumn derivatization
The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.
The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.
|AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization Specifications|
AminoPac PA1 Analytical Column:
4 × 250 mm
AminoPac PA1 Guard Column:
4 × 50 mm
|Maximum operating pressure||4000 psi (27.9 MPa)|
|Recommended operating temperature||Ambient|
|Typical operating conditions||
1.0 mL/min gradient
Recommended flow rate: 1.0 mL/min
|Mobile phase compatibility||
Compatible with up to 5% common organic solvents.
Recommended eluents: hydroxide, borate, and acetate.
10-μm-diameter, pellicular substrate (2% cross-linked)
agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
Gradient sepration of amino acid hydrolysate.
Amino Acids by Anion Exchange Using The AminoPac® PA1 Column.
Anion-Exchange Separation of O-Phosphorylated Amino Acids Using the AminoPac® PA1 Column.
|AminoPac PA1 Analytical Column Manual|
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